c freundii strain atcc 8090  (ATCC)

Journal: iScience

Article Title: Biosynthesis of polyamine-polyphosphate granules for colitis alleviation

doi: 10.1016/j.isci.2026.114679

Figure Lengend Snippet: Two-step cultivation of engineered C. freundii promotes PolyP accumulation and granule formation (A and B) Morphological analysis of intracellular granules in engineered C. freundii (CPP) cultured under Two-step and Luria-Bertani (LB) medium conditions. Representative images from optical microscopy and transmission electron microscopy (TEM) are shown. Scale bars: 800 nm (optical) and 300 nm (TEM). (C) Phosphate uptake by CPP shows enhanced PolyP synthesis under Two-step cultivation ( n = 3).

Article Snippet: The wild-type Citrobacter freundii ATCC 8090 strain was obtained from the China Industrial Microbiology Culture Collection ( ).

Techniques: Cell Culture, Microscopy, Transmission Assay, Electron Microscopy

Journal: iScience

Article Title: Biosynthesis of polyamine-polyphosphate granules for colitis alleviation

doi: 10.1016/j.isci.2026.114679

Figure Lengend Snippet: Two-step cultivation of engineered C. freundii promotes PolyP accumulation and granule formation (A and B) Morphological analysis of intracellular granules in engineered C. freundii (CPP) cultured under Two-step and Luria-Bertani (LB) medium conditions. Representative images from optical microscopy and transmission electron microscopy (TEM) are shown. Scale bars: 800 nm (optical) and 300 nm (TEM). (C) Phosphate uptake by CPP shows enhanced PolyP synthesis under Two-step cultivation ( n = 3).

Article Snippet: Citrobacter freundii ATCC 8090 , American Type Culture Collection , ATCC 8090.

Techniques: Cell Culture, Microscopy, Transmission Assay, Electron Microscopy

Journal: bioRxiv

Article Title: Programmable RNA detection generates DNA barcodes for multiplexed phage-host interaction screening

doi: 10.64898/2026.02.13.705812

Figure Lengend Snippet: a , Schematic of the Detectron variant library design. stem1, stem2, bulge, loop, and the distance from stem base to the priming guanosine (d) were systematically varied to construct 7,840 variants. b , Schematic of a barcoded-Detectron variant: each variant was linked to three unique DNA barcodes encoded in the msd loop, expanding the library to 23,520 constructs. c , Design of synthesized oligos and Golden Gate cloning workflow for constructing the variant library. d , Distribution of ON/OFF ratios across the variant library, ranked from highest to lowest ON/OFF ratio. Each circle represents a Detectron variant, color-coded by the average ON/OFF ratios (±s.d.) of three biological replicates. V1, original Detectron design. e , Scatter plot of ON-state versus OFF-state signals across all Detectron variants. Points are color-coded by ON/OFF ratio. f , Structural features and parameter values of the top three Detectron variants with the highest the average ON/OFF ratios. g , Urea PAGE visualization of RT-DNA from No Toehold control (lane 1, 4, 7, 10, excluding ladder) and the indicated Detectron variants in the absence (lane 2, 5, 8, 11) or presence of trigger RNA expressed under the J23100 promoter (lane 3, 6, 9, 12). h , Enrichment of RT-DNA + plasmid over plasmid alone, as measured by qPCR; One-way ANOVA with Tukey’s multiple comparisons test (corrected) for OFF-state signal: #621 OFF versus V1 OFF: P < 0.0001; #121 OFF versus V1 OFF: P < 0.0001; #61 OFF versus V1 OFF: P < 0.0001; One-way ANOVA with Tukey’s multiple comparisons test (corrected) for ON-state signal: #621 ON versus V1 ON: P < 0.0001. Closed circles indicate four biological replicates. Bars represent the mean (±s.d.) of four biological replicates. i , ON/OFF ratios of indicated Detectron variants; One-way ANOVA with Tukey’s multiple comparisons test (corrected): #621 versus V1: P < 0.0001; #121 versus V1: P < 0.0001. Bars represent the mean (±s.d.) of four biological replicates. j , Urea PAGE visualization of RT-DNA from V1 and #621 Detectrons in the absence (lane 1, 3) or presence of trigger RNA expressed under the J23100 promoter (lane 2, 4) in K. pneumoniae (left) and C. freundii (right).

Article Snippet: Klebsiella pneumoniae (ATCC 10031) and Citrobacter freundii (ATCC 8090) were used to assess portability of Detectron-V2.

Techniques: Variant Assay, Construct, Synthesized, Cloning, Control, Plasmid Preparation